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tmb (Thermo Fisher)

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Thermo Fisher tmb
Tmb, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tetramethylbenzidine+tmb/3%2C3'%2C5%2C5'-Tetramethylbenzidine+soln%2E%2C+Ready-to-Use%2C+high+sensitivity/pm42330958-321-4-11
Average 95 stars, based on 1 article reviews

tmb - by Bioz Stars, 2026-11

95/100 stars

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Article Title: Development and characterization of the genotype F attenuated mumps candidate strains
Article Snippet: Tetramethylbenzidine (TMB) (Thermo, XB3498751) was then added as a substrate, and the reaction was quenched by adding the stop solution for TMB.

Article Title: Scaffold vaccination for prevention of orthopedic device infection
Article Snippet: Wells were washed ×3 and 100 μL of 3,3′,5,5′-tetramethylbenzidine (TMB)-detecting HRP buffer was added to each well (ThermoScientific).

Article Title: Scaffold vaccination for prevention of orthopedic device infection.
Article Snippet: Plates were then washed ×3 with working buffer and blocked by incubating with 1:20 pig sera (VWR) for 90 min at room temperature on an orbital shaker at 300 RPM.

Article Title: Nanoparticle vaccine based on the pre-fusion F glycoprotein of respiratory syncytial virus elicits robust protective immune responses.
Article Snippet: After six additional washes with PBST, the plates were developed using tetramethylbenzidine (TMB) (Thermo Fisher Scientific, USA) for 10 min at RT.

BIA-KA:

Article Title: Effect of Acute Lung Injury (ALI) Induced by Lipopolysaccharide (LPS) on the Pulmonary Pharmacokinetics of an Antibody.
Article Snippet: The primary and secondary antibodies for ELISA were purchased from Bethyl Laboratory (Montgomery, TX, USA). .. BCA Assay kit, 384-well plates, para-nitrophenyl phosphate (PNPP), and tetramethylbenzidine (TMB) reagents were purchased from Thermo Scientific (Waltham, MA, USA). ..

Article Title: Effect of Acute Lung Injury (ALI) Induced by Lipopolysaccharide (LPS) on the Pulmonary Pharmacokinetics of an Antibody
Article Snippet: The primary and secondary antibodies for ELISA were purchased from Bethyl Laboratory (Montgomery, TX, USA). .. BCA Assay kit, 384-well plates, para-nitrophenyl phosphate (PNPP), and tetramethylbenzidine (TMB) reagents were purchased from Thermo Scientific (Waltham, MA, USA). ..

Binding Assay:

Article Title: Catch, Cut, or Block? Versatile 4‐ N ‐Derivatized Sialyl Glycosides for Influenza Virus Neuraminidase Detection and Purification
Article Snippet: PierceTM streptavidin and NeutrAvidin-coated plates were purchased from Thermo Fisher Scientific. .. The binding was detected using a horseradish peroxidase (HRP)-conjugated anti-His6 monoclonal antibody (ThermoFisher Scientific) or HRP-conjugated antiStrepTag II monoclonal antibody (Sigma-Aldrich), and a colorimetric substrate solution 3,3’,5,5’- tetramethylbenzidine (TMB) (ThermoFisher Scientific). .. Pierce streptavidin and NeutrAvidin-coated plates were purchased from Thermo Fisher Scientific.

Incubation:

Article Title: Defined Glycan Ligands for Detecting Rare l -Sugar-Binding Proteins
Article Snippet: Then, the primary antibody, mouse anti-human blood group antigen H (O) Type 1 IgG3 mAb (Invitrogen) or mouse anti-human blood group antigen H (O) Type 2 IgM mAb (Bio-Techne), was added at 8 different dilutions (starting with 10 or 4 μg/mL to 0, respectively) in blocking buffer B; each well was incubated with 50 μL of diluted primary antibodies or blocking buffer B for blank at 25 °C for 1.5 h. After washing the wells (3 x 200 μL TBS-T, 5 min each), 50 μL of secondary antibody, goat anti-mouse IgG HRP (1:10K, BioLegend) or goat anti-mouse IgM HRP (1:50K, Sigma Aldrich) respectively, in blocking buffer B was incubated for 1.5 h at 25 °C. .. After washing the wells (6 x 200 μL TBS-T, 5 min each), 50 μL of tetramethylbenzidine (TMB, Thermo Fisher) was added to each well and incubated at 25 °C for 4 min or 30 min respectively, before quenching with 50 μL of 2 M sulfuric acid. .. Absorbance of each well was monitored at 450 nm using a plate reader (SpectraMax iD5, Molecular Devices) prior to analysis in SoftMax Pro 7.1 and Graphpad PRISM.